作者：邹忠香，徐杰，沙秀敏，张素华 作者单位：1 265200 山东，烟台市莱阳中心医院妇产科 2 山东，蓬莱市人民医院普外科
【摘要】 目的 研究表皮生长因子受体(epidermal growth factor receptor,EGFR)基因和细胞角质蛋白20(cytokeratin-20，CK20)在卵巢上皮性肿瘤中的表达及意义。方法 应用RT-PCR法检测12例正常卵巢(对照组)、18例卵巢良性肿瘤(良性组)、33例卵巢上皮癌(恶性组)组织中EGFR、CK20 mRNA的表达，分析二者与卵巢肿瘤发生发展及转移的关系。结果 (1)原发性卵巢上皮癌组织中EGFR和CK20 mRNA的阳性表达率显著高于正常卵巢及卵巢良性肿瘤组织(P<0.05);(2)EGFR mRNA表达与原发性卵巢上皮癌手术病理分期有关，其在Ⅲ～Ⅳ期卵巢癌患者组织中的表达阳性率高于Ⅰ～Ⅱ期患者组织(P<0.05);CK20 mRNA表达与卵巢上皮癌手术病理分期、淋巴结转移有关(P<0.05)，其在Ⅲ～Ⅳ期和有淋巴结转移组的患者组织中阳性表达率分别高于Ⅰ～Ⅱ期和无淋巴结转移组患者(P<0.05);(3)EGFR与CK20基因表达之间有显著相关性(P<0.05)。结论 (1)EGFR mRNA和CK20 mRNA在卵巢癌组织中有较高的表达，可能与卵巢癌发生、发展和转移有关;(2)检测卵巢癌组织中EGFR mRNA、CK20 mRNA的阳性表达对判断卵巢癌患者的转移和估计预后有一定的参考价值。
【关键词】 卵巢肿瘤;表皮生长因子受体;细胞角质蛋白20;逆转录聚合酶链式反应
Expression and significance of EGFR and CK-20 in ovarian carcinoma
ZOU Zhong-xiang,XU Jie,SHA Xiu-min，et al.Department of Obstetrics and Gynecology,Shangdong Province Yantai City Laiyang Central Hospital,Yantai 265200,China
[Abstract] Objective To investigate the expression and significance of epidermal growth factor receptor (EGFR) and cytokeratin-20 (CK20) in ovarian epithelial tumor,and to explore their roles in the tumorigenesis,development and metastasis of ovarian tumor.Methods The expression of EGFR and CK20 were detected by reverse transcription polymerase chain reaction (RT-PCR) in 12 cases of normal ovary tissue,18 cases of benign ovarian tumor and 33 cases of primary ovarian carcinoma.The relationship between the two genes and the different clinicopathological features were analyzed.Results (1)The positive rates of EGFR mRNA and CK20 mRNA were significantly higher in malignant ovarian specimens than in the normal and benign ones (P<0.05).(2) The expression of EGFR mRNA was correlated to clinical stage in primary ovarian carcinoma,EGFR mRNA in the patients with stage Ⅲ～Ⅳ had higher positive expression rate than that in stageⅠ～Ⅱ(P<0.05).The expression of CK20 mRNA in ovarian carcinoma was related with the clinical stage and lymph metastasis (P<0.05).CK20 mRNA expression rates in patients with stage Ⅲ～Ⅳ and in the group with lymph node metastasis were significantly higher than those in stageⅠ～Ⅱ and in the group without lymph node metastasis respectively(P<0.05).(3) There was distinct correlation between EGFR mRNA and CK20 mRNA expression (P<0.05).Conclusion (1) The expressions of EGFR mRNA and CK20 mRNA are higher in ovarian cancer and may be correlated to the occurrence,development and metastasis of ovarian carcinoma.(2) Detection of EGFR mRNA and CK20 mRNA can offer a valuable reference to evaluate the metastasis and prognosis in ovarian carcinoma.
[Keywords] ovarian carcinoma;epidermal growth factor receptor;cytokeratin-20;reverse transcription polymerase chain reaction
原发性卵巢癌是世界范围内妇女常见的恶性肿瘤，复发和转移是主要致死因素。近20年来卵巢癌的发病率有上升的趋势，其病死率已居各类妇科肿瘤的首位[1]。卵巢癌具有临床发现晚、转移早、治疗效果不好等特点，多数患者就诊时已处于疾病的晚期阶段。研究和寻找有关卵巢癌诊断、转移及复发的生物学标记物对于指导治疗、提高术后生存率有十分重要的意义。本研究应用逆转录聚合酶链式反应(RT-PCR)检测表皮生长因子受体(EGFR)与细胞角蛋白20(CK20)在不同卵巢肿瘤中的表达，探讨作为卵巢癌肿瘤细胞标志的可能性,并分析两者的相关性意义。